GRHL3 Promotes Tumor Growth and Metastasis via the MEK Pathway in Colorectal Cancer

Figure 4

Decreased GRHL3 expression represses cell proliferation and invasion. (a) The protein expression of β-catenin, ERK1/2, p-ERK1/2, Vimentin, and E-cadherin was assayed by western blotting. (b) Cytoskeletal staining of F-actin. (c-e) Cell migration and invasion were detected by transwell in different treatment cells. (f, g) Vimentin and E-cadherin were assayed using immunofluorescence staining. Stably transfected knockdown expression of GRHL3 (GRHL3-KD) and negative control (NC) HT29 cells was treated with U0126 or solvent (vehicle).

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