miR-486 Promotes the Invasion and Cell Cycle Progression of Ovarian Cancer Cells by Targeting CADM1

Figure 4

miR-486 downregulates CADM1 to be involved in the biological function of OC cells. (a) CCK-8 assay to detect the cell viability of SKOV3 after transfection with in-NC+si-NC, in-miR-486+si-NC, and in-miR-486+si-CADM1; (b) colony formation assay to detect the cell colony formation ability of SKOV3 after transfection with in-NC+si-NC, in-miR-486+si-NC, and in-miR-486+si-CADM1; (c) Transwell assay to detect the invasion ability of SKOV3 after transfection with in-NC + si-NC, in-miR-486+si-NC, and in-miR-486+si-CADM1; (d, e) FCM to detect the apoptosis rate and cell cycle of SKOV3 after transfection with in-NC+si-NC, in-miR-486+si-NC, and in-miR-486+si-CADM1; (f) Western blot to detect the expression levels of cell cycle-related proteins p21, CyclinD1, CyclinE, and CDK6. and vs. the in-NC+si-NC group; ^# and ^## vs. the in-miR-486+si-NC group.

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