Research Article

The Synergistic Effect of TRPV1 on Oxidative Stress-Induced Autophagy and Apoptosis in Microglia

Figure 4

TRPV1 and apoptosis, Atg5-mediated autophagy in OGD/R microglia. Apoptosis was assayed in OGD/R microglia, and an autophagy-related protein of siRNA-Atg5 (siAtg5) along with negative (NC) siRNA-transfected cells, TRPV1 agonist Cap, and TRPV1 inhibitor iRTX was involved. (a, b) JC-1 was assayed by flow cytometry and analyzed. (c) The level of caspase 3 was measured using the ELISA kit. (d) Apoptosis was assayed using TUNEL staining. The data was given as , . and compared with microglia in culture (control). compared with microglia on glucose depreciation/reoxygenation (OGD/R) culture condition (OGD/R).
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