MicroRNA-520a Suppresses Pathogenesis and Progression of Non-Small-Cell Lung Cancer through Targeting the RRM2/Wnt Axis

Figure 3

miR-520a directly binds to RRM2 to mediate the Wnt signaling pathway. (a) A Venn diagram for the intersected target mRNAs of miR-520a predicted on StarBase, TargetScan, miRDB, and miRbase. (b) The enriched signaling pathway by the putative mRNAs identified by GO enrichment analysis. (c) Protein levels of cyclin D1 and β-catenin in A549 and H460 cells after miR-520a mimic transfection determined by western blot analysis (, two-way ANOVA). (d) The expression of Wnt-enriched mRNAs in NSCLC tumor tissues determined by RT-qPCR (, two-way ANOVA). (e) Binding relationships between miR-520a and SDC1, YWHAZ, or RRM2 validated through dual-luciferase reporter gene assays (, two-way ANOVA). (f) Correlation analysis of the RRM2 and miR-520a expression in tumor tissues. (g) The RRM2 expression in NSCLC cell lines H273, H23, A549, and H460 and in normal pulmonary epithelial cell line 16HBE determined by RT-qPCR (, one-way ANOVA). (h) The RRM2 expression in A549 and H460 cells after miR-520a mimic transfection detected by RT-qPCR (, two-way ANOVA). (i) Protein levels of cyclin D1 and β-catenin in A549 and H460 cells after the RRM2 overexpression determined by western blot analysis (, two-way ANOVA).

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