MYC Promotes LDHA Expression through MicroRNA-122-5p to Potentiate Glycolysis in Hepatocellular Carcinoma

Figure 4

miR-122-5p hinders glycolysis in HCC cells through targeting LDHA. (a) Prediction of the targeting sites between miR-122-5p and LDHA 3UTR. (b) The binding relation between miR-122-5p and LDHA verified using dual-luciferase reporter assays. (c) LDHA expression in clinical tissues using RT-qPCR. (d) LDHA expression in NHC cells and HCC cells MHCC97H, Huh7, Hep3B using RT-qPCR. (e) LDHA expression by RT-qPCR and western blot after restoration of miR-122-5p in MHCC97H and Huh7 cells. (f) Detection of LDHA/LDHB ratio in MHCC97H and Huh7 cells using ELISA. (g)–(i) Glucose consumption (g), lactate production (h), and ATP contents (i) in MHCC97H and Huh7 cells measured by their respective kits. (j) The protein expression of HK1 and HK2 in MHCC97H and Huh7 cells evaluated using Western blot. vs. NC mimic, NHC cells, or NC mimic + oe-NC; # vs. miR-122-5p mimic + oe-NC. Statistical results were presented by . Paired -test and one-way or two-way ANOVA were used for comparisons. Each reaction was run in triplicate.

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