miR-1266-3p Suppresses Epithelial-Mesenchymal Transition in Colon Cancer by Targeting P4HA3

Figure 2

P4HA3 was a direct target of miR-1266-3p. (a) Volcano map of differentially expressed genes in normal and tumor groups. The red ball represents upregulated gene and the blue ball mean downregulated gene. (b) Venn diagram of upregulated genes and predicted target genes. (c) A schematic diagram of miR-1266-3p at the 3’-UTR predicted binding site of P4HA3 mRNA in the Targetscan database. (d) The correlation of miR-1266-3p with P4HA3 in the GSE35843 dataset was analyzed using the Pearson’s method (, ). (e) Schematic diagram of the luciferase reporter vector construction for wild-type P4HA3 3’UTR (P4HA3 3’UTR-Wt) and miR-1266-3p-binding site mutation (P4HA3 3’UTR-Mut). (f) Luciferase reporter assay of HEK293 cells cotransfected with constructed luciferase reporter vectors (P4HA3 3’UTR-Wt, P4HA3 3’UTR-Mut, empty Vector) and miR-1266-3p mimics or negative control mimics (miR-NC) ().

(a)

(b)

(c)

(d)

(e)

(f)