MicroRNA-147a Targets SLC40A1 to Induce Ferroptosis in Human Glioblastoma

Figure 4

The miR-147a mimic elicits ferroptosis of human glioblastoma cells through targeting SLC40A1. (a) Sequence alignment of miR-147a and the 3-UTR of SLC40A1. (b) SLC40A1 protein level in human glioblastoma tissues and normal brain tissues. (c–e) The mRNA and protein levels of SLC40A1 in cells treated with or without the miR-147a mimic. (f) Relative luciferase activity. (g) The mRNA level SLC40A1 in cells infected with or without AdSCL40A1. (h) Cell viability detected by CCK-8 assay. (i) LDH level in the medium from the miR-147a mimic-treated cells with or without SLC40A1 overexpression. (j) Relative level of intracellular iron. for each group. Data were expressed as the , and was considered statistically significant. NS indicates no significance.

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