XBP1 Regulates the Transcription of HIF-1a in BALB/c Mice with Chronic Rhinosinusitis without Polyps

Figure 3

XBP1 promotes the expression of HIF-1a. (a) HIF-1a was predicted as a downstream gene of XBP1 in the hTFtarget database. (b) Potential binding sites of the HIF-1a promoter to XBP1. (c) RT-qPCR analysis of HIF-1a expression in nasal mucosal tissues from CRSsNP patients and controls. (d) RT-qPCR analysis of HIF-1a expression in nasal mucosal tissues from CRSsNP mice and controls. (e) RT-qPCR and (f) Western blot analyses of XBP1 mRNA and protein expression in mice. (g) Detection of mRNA expression of XBP1 and HIF-1a in cells by RT-qPCR. (h) The enrichment ability of anti-XBP1 on HIF-1a promoter assessed using ChIP-qPCR. (i) The effect of overexpression of XBP1 on promoter luciferase activity of HIF-1a measured using dual-luciferase assay. (j) The effect of overexpression of XBP1 on HIF-2a and HIF-3a mRNA expression by RT-qPCR assay. (k) The effect of overexpression of XBP1 on HIF-2a and HIF-3a protein expression by Western blot assay. and versus the CRSsNP mice administrated with sh-NC or HNEPC transfected with oe-NC. The data are measurement data, presented as . Data between two groups were compared using an unpaired test. Data among multiple groups were compared using two-way analysis of variance and then analyzed with Tukey’s post hoc test. The experiment was independently repeated three times. .

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