XBP1 Regulates the Transcription of HIF-1a in BALB/c Mice with Chronic Rhinosinusitis without Polyps

Figure 5

Overexpression of HIF-1a reverses the alleviating effect of sh-XBP1 on the development of CRSsNP in mice. (a, b) Analysis of mRNA and protein expression of XBP1 and HIF-1a after further overexpression of HIF-1a in CRSsNP mice with silenced XBP1 expression by (a) RT-qPCR and (b) Western blot. (c) PAS staining analysis of mucin expression in nasal mucosal epithelial cells of mice. (d) Pathological changes of nasal mucosa in mice observed by HE staining. (e) The levels of inflammatory factors IFN-γ, IL-1β, and IL-6 in nasal mucosal tissues of mice measured using ELISA. (f) The levels of inflammatory factors IL-4 and IL-13 in nasal mucosal tissues of mice measured using ELISA. (g) Western blot detection of protein expression of β-catenin in nasal mucosal tissues of CRSsNP mice. and versus the CRSsNP mice administrated with sh-XBP1+oe-NC. The data are measurement data, presented as . Data between two groups were compared using an unpaired test. Data among multiple groups were compared using one-way analysis of variance and then analyzed with Tukey’s post hoc test. The experiment was independently repeated three times. .

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