Metformin Protects against Spinal Cord Injury and Cell Pyroptosis via AMPK/NLRP3 Inflammasome Pathway

Figure 5

Metformin activates the AMPK/NLRP3 signaling pathway and inhibits inflammation after SCI. (a–c) Representative Western blots and quantification data of the levels of p-AMPK and p-ACC after SCI from each group. All data represent the , , . (d–h) Representative Western blots and quantification data of the expression of NLRP3, ASC, Caspase 1, and IL-1β after SCI from each group. All data represent the , , . (i, j) Immunohistochemical detection of the levels of NLRP3 protein in each group of rats at 7 days after SCI. Scale bars represent 100 μm. , . (k–m) ELISA was used to detect the expression of IL-1β, IL-6, and TNF-α in the spinal cord tissue homogenate from each group. All data represent the , , .

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