Reduction of Activin Receptor-Like Kinase 4 Expression Ameliorates Myocardial Ischemia/Reperfusion Injury through Inhibiting TGFβ Signaling Pathway

Figure 1

Serum levels of related factors in different groups: WT, ALK4-NC, ALK4-NC+I/R, and ALK4-KO+I/R groups. (a) The hydroxylamine method was used to detect SOD level at 24 h after I/R. (b) The thiobarbituric acid method was employed to detect MDA level at 24 h after I/R. (c–e) Serum levels of CK, LDH, and AST were assessed with corresponding ELISA kits at 24 h after I/R. (f–h) Inflammation related factors (IL-6, TNF-α, and IL-1β) were detected with corresponding ELISA kits at 24 h after I/R. (i and j) The serum levels of PG12 and TXA2 were detected with corresponding ELISA kits at 24 h after I/R. (k) Representative images of nonischemic zones (blue) and infracted tissue (white) within the AAR (absence of blue dye). Data are expressed as . , , and , WT+I/R group vs. WT+sham group. ^#, ^##, and ^###, ALK4^+/-+I/R group vs. WT+I/R group.

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