Reduction of Activin Receptor-Like Kinase 4 Expression Ameliorates Myocardial Ischemia/Reperfusion Injury through Inhibiting TGFβ Signaling Pathway

Figure 2

Detection of cell aging, cell apoptosis, and related molecules in different groups: WT+sham group, WT+I/R group, ALK4^+/-+sham group, and ALK4^+/-+I/R group. (a) HE staining of cell aging. (b and c) Detection of protein expressions of TGFβ, AKT1, STAT3, and NF-κB (Western blotting). (d–g) Detection of protein expressions of phosphorylated Smad2/3 (p-Smad2/3), Smad4, Smad6, and Smad7 (Western blotting). (h) Green: apoptotic cells (TUNEL staining); blue: nuclei (DAPI counterstaining, ×400). and , WT+I/R group vs. WT+sham group. ^# and ^##, ALK4^+/-+I/R group vs. WT+I/R group.

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