Reduction of Activin Receptor-Like Kinase 4 Expression Ameliorates Myocardial Ischemia/Reperfusion Injury through Inhibiting TGFβ Signaling Pathway

Figure 3

Expression of molecules related to TGF-β signaling pathway, antioxidants, inflammation-associated cytokines, and apoptosis-related molecules at 24 h after I/R and another 72 h after H9C2 cells were transfected with shRNA-TGF-β. (a and b) Expression of TGFβ, p-Smad2/3, Smad4, Smad6, and Smad7 in the cells from the control group and I/R group at 24 h after I/R (Western blotting). (c) The thiobarbituric acid method was employed to detect MDA level. (d) The hydroxylamine method was used to detect SOD. (e–g) Serum levels of CK, LDH, and AST were detected with corresponding ELISA kits. (h–j) Inflammation-related factors (IL-1β, TNF-α, and IL-6) were detected with corresponding ELISA kits. (k and l) Expression of BCL-2, Bax, and caspase-3 (Western blotting). Data are presented as . , , and , IR group vs. NC group. ^#, ^##, and ^###, IR-sh TGF-β group vs. IR group.

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