Research Article
Reduction of Activin Receptor-Like Kinase 4 Expression Ameliorates Myocardial Ischemia/Reperfusion Injury through Inhibiting TGFβ Signaling Pathway
Figure 6
Effects of ALK4 on TGF-β protein stability and transcriptional activity in the H9C2 cells experiencing I/R injury. (a) CHX was used to inhibit protein synthesis. The cells were lysed and total proteins were extracted for Western blotting with anti-TGF-β antibody. (b) Coimmunoprecipitation assay of Ub protein expression. (c) Luciferase report assay was carried out to assess the transcriptional activity of TGF-β promoter. Data are presented as . , , and , ALK4-NC+I/R group vs. ALK4-NC group. #, ##, and ###, ALK4-KO+I/R group vs. ALK4-NC+I/R group.
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