Reduction of Activin Receptor-Like Kinase 4 Expression Ameliorates Myocardial Ischemia/Reperfusion Injury through Inhibiting TGFβ Signaling Pathway

Figure 8

In the cell model of hypoxia/reoxygenation, TGF-β was knocked out, and exogenous ALK4 protein was added. HE staining of heart tissues. The aging condition: and . Evans blue and TTC staining found no significant change in myocardial infarction area after addition of TGF-β, followed by detection of SOD, MDA, CK, LDH, AST, IL-6, TNF-α, and IL-1β. There was no significant difference between ALK4^+/-+I/R and TGF-β groups, and it was significantly higher than the ALK4^+/-+sham groups. (a–h) Antioxidation-related indicators and inflammation-related indicators TNF-α, IL-6, and IL-1. (i) HE staining. (j) Evans Blue and TTC double staining.

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