YAP Overexpression in Breast Cancer Cells Promotes Angiogenesis through Activating YAP Signaling in Vascular Endothelial Cells

Figure 5

Connective tissue growth factor and angiopoietin-2 acted as down-stream of YAP in HUVECs to promote tube formation. (a) HUVECs were starved for 16 hr, then, treated with CM for 12 hr. The mRNA levels of CTGF and ANG-2 in HUVECs were determined by quantitative real-time PCR. Normalized expressions values are were quantified with from three independent experiments . . (b) HUVECs were starved for 16 hr, then, treated with CM for 12 hr. The protein levels of CTGF and ANG-2 in HUVECs were determined by Western blot. Representative results are shown from three independent experiments. . (c) HUVECs were transfected with different siRNAs for 48 hr, starved and then, treated with CM for 12 hr. The total segment length and total branching length from tube formation assay were quantified with from six independent representative fields. . .

(a)

(b)

(c)