Magnetic Resonance Imaging of Contrast-Induced Acute Renal Injury and Related Pathological Alterations In Vivo

Figure 7

Protein related to CI-AKI. (a) Serum NGAL. A dramatic increasing of NGAL presents at 24 h after CM injection. (b) Western blotting of the kidney. After administration of CM for 48 h, the expression of Nlrp3 and pro-caspase-1 got a marked increase. NF-κB, caspase-8, CytoC, and BAX got a moderate increase. On the contrary, Bcl-2 got a moderate decrease. BNIP3L/Nix, which is related to autophagy and mitophagy, also got an obvious increase. GAPDH is internal control. (c–k) Relative densitometry analysis; and . Nlrp3: nucleotide oligomerization domain-like receptor pyrin 3; NF-κB: nuclear factor kappa-B; cas8: caspase8; pro-cas1: pro-caspase1; BNIP3L/Nix: Bcl2 interacting protein3/Nip3-like protein; Bcl2: B-cell lymphoma-2; BAX: Bcl2-associated X; CytoC: cytochrome C; GAPDH: glyceraldehyde-3-phosphage dehydrogenase; nor: normal; CM: contrast media; nor: normal.

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