Transcription Factor E2F1 Exacerbates Papillary Thyroid Carcinoma Cell Growth and Invasion via Upregulation of LINC00152

Figure 2

E2F1 induces the upregulation of LINC00152 expression. (a) E2F1 expression patterns in PTC cell lines measured by RT-qPCR. (b) Binding site between E2F1 and LINC00152 promoter predicted by JASPAR. (c, d) The interaction between E2F1 and LINC00152 in (c) TPC-1 and (d) IHH-4 cells verified by RIP. (e, f) The interaction between E2F1 and LINC00152 in (e) TPC-1 and (f) IHH-4 cells detected by dual-luciferase reporter gene assay. (g, h) Expression patterns of E2F1 and LINC00152 in (g) TPC-1 and (h) IHH-4 cells after silencing E2F1 assessed by RT-qPCR. . Data are expressed as . The -test was used for pairwise comparison. One-way ANOVA was used to determine statistical significance. Tukey’s multiple comparisons test was applied for post hoc test. ; .

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