miR-590-5p Overexpression Alleviates β-Amyloid-Induced Neuron Damage via Targeting Pellino-1

Figure 3

Effect of miR-590-5p overexpression on PEIL1 in Aβ-induced BV-2 and HT22 cells. (a) The mRNA levels of miR-590-5p and PELI1 in BV-2 or HT22 cells treated with Aβ (AD model), Aβ+vector, and Aβ+miR-590-5p mimics, respectively, by qPCR. (b) The protein levels of PELI1 in BV-2 or HT22 cells treated with Aβ (AD model), Aβ+vector, and Aβ+miR-590-5p mimics, respectively, by western blotting. (c) The PELI1 expression in BV-2 or HT22 cells treated with Aβ (AD model), Aβ+vector, and Aβ+miR-590-5p mimics, respectively, by cell immunofluorescence. Each experiment was repeated three times. , vs. control group; ^### vs. AD model+vector group.

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