miR-590-5p Overexpression Alleviates β-Amyloid-Induced Neuron Damage via Targeting Pellino-1

Figure 4

Effect of miR-590-5p overexpression on cell viability, apoptosis, and Traf3/MAPK P38 pathway in Aβ-induced BV-2 and HT22 cells. (a) Cell viability of BV-2 or HT22 cells treated with Aβ (AD model), Aβ+vector, and Aβ+miR-590-5p mimics, respectively, by CCK-8 assay. (b) The cell apoptosis of BV-2 or HT22 cells treated with Aβ (AD model), Aβ+vector, and Aβ+miR-590-5p mimics, respectively, by TUNEL assay (blue, nucleus; green, TUNEL-positive cells). (c, d) The expression of Traf3, phosphorylation levels of ERK1/2 and P38, and apoptosis-related proteins (Bcl-2 and Bax) in BV-2 or HT22 cells treated with Aβ (AD model), Aβ+vector, and Aβ+miR-590-5p mimics, respectively, by western blotting. Each experiment was repeated three times. vs. control group; ^### vs. AD model+vector group.

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