miR-590-5p Overexpression Alleviates β-Amyloid-Induced Neuron Damage via Targeting Pellino-1

Figure 6

Effect of miR-590-5p silence on BV-2 and HT22 cells was regulated by PELI1. (a, b) The mRNA and protein levels of PELI1 in BV-2 or HT22 cells treated with vector, PELI1 (overexpression of PELI1 CDS+3UTR), and PELI1 (overexpression of PELI1 CDS+3UTR)+miR-590-5p mimics, respectively, by qPCR and western blotting. (c, d) Cell viability of BV-2 or HT22 cells treated with vector, PELI1, and PELI1+miR-590-5p mimics, respectively, by CCK-8 assay. (e) The cell apoptosis of BV-2 or HT22 cells treated with vector, PELI1, and PELI1+miR-590-5p mimics, respectively, by TUNEL assay (blue, nucleus; green, TUNEL-positive cells). (f) The expression of Traf3, phosphorylation levels of ERK1/2 and P38, and apoptosis-related proteins (Bcl-2 and Bax) in BV-2 or HT22 cells treated with vector, PELI1, and PELI1+miR-590-5p mimics, respectively, by western blotting. Each experiment was repeated three times. vs. vector group; ^### vs. PELI1 group.

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