circ_0052184 Promotes Colorectal Cancer Progression via Targeting miR-604/HOXA9 Axis

Figure 5

circ_0052184 regulated HOXA9 expression by targeting miR-604. (a) starBase was used to predicted the complementary sequence between miR-604 and HOXA9 3UTR. (b) Dual-luciferase reporter gene assay was used to confirm the binding relationship between miR-604 and HOXA9. (c, d) HOXA9 mRNA and protein level were measured by qRT-PCR (c) and Western blot (d) in CRC tissues. (e, f) HOXA9 mRNA and protein levels were measured by qRT-PCR (e) and Western blot (f) in CRC cell lines. (g) The protein expression of HOXA9 was measured by Western blot in CRC cells transfected with control siRNA (si-NC), circ_0052184 siRNA (si-circ_0052184), or cotreated with si-circ_0052184 and inhibitors of miR-604, respectively. (h) Pearson correlation between HOXA9 mRNA expression and miR-604 expression in CRC tissues. , , .

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