Mesenchymal Stem Cells and their Derived Exosomes Promote Malignant Phenotype of Polyploid Non-Small-Cell Lung Cancer Cells through AMPK Signaling Pathway

Figure 2

Doc induced formation of the polyploid NSCLC cells. (a) Experimental design. The NSCLC cell lines A549 and H1299 were treated with 1/0.5 μM Doc for 24 hours to induce mitotic failure and then cultured in a drug-free medium for 3 days. Human umbilical cord-derived MCSs, MSC-CM, MSC-exosomes, and the polyploid A549 or H1299 cells were cultured together for 48 hours or 72 hours in indirect co-culture using the transwell system. (b) Morphologic change in A549 and H1299 cells by conventional light microscopy after treatment with1/0.5 μM Doc for 24 hours and then cultured in a drug-free medium for 3 days (×200). (c) The morphology of the polyploid A549 and H1299 cells were observed by fluorescence microscopy; blue represented DAPI-labeled nucleus (×200). (d, e) DNA content of the polyploid A549 and H1299 cells was analyzed by flow cytometry.

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