Mesenchymal Stem Cells and their Derived Exosomes Promote Malignant Phenotype of Polyploid Non-Small-Cell Lung Cancer Cells through AMPK Signaling Pathway

Figure 4

MSCs and MSC-exosomes promote migration and epithelial-mesenchymal transformation in the polyploid A549 and H1299 cells. (a, b) The CFSE proliferation analysis of the polyploid A549 and H1299 cells following coculture with MSC-CM, MSCs, and MSC-exosomes was examined by flow cytometry at 0 hour, 48 hours, and 72 hours. (c, d) The quantitative analysis of migration ability of the polyploid A549 and H1299 cells following coculture with MSC-CM, MSCs, and MSC-exosomes was examined by transwell assay at 48 hours. (e) The expressions of ECAD, NCAD, and VIM in the polyploid A549 and H1299 cells following c-culture with MSC-CM, MSCs, and MSC-exosomes were detected by RT-qPCR analysis at 48 hours.

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