Extracellular Vesicle-Encapsulated MicroRNA-375 from Bone Marrow-Derived Mesenchymal Stem Cells Inhibits Hepatocellular Carcinoma Progression through Regulating HOXB3-Mediated Wnt/β-Catenin Pathway

Figure 1

miR-375 expression is reduced in HCC, and overexpression of miR-375 retards the HCC occurrence. (a) miR-375 expression in HCC tissues and their corresponding paracancerous tissues of 13 patients with HCC was detected by RT-qPCR. (b) The correlation between miR-375 and patients’ survival in the TCGA-LIHC database analyzed using Kaplan-Meier analysis. (c) miR-375 expression in the cells after transfection using RT-qPCR. (d) Cell proliferation in HCC cells using EdU assay; (e). Cell apoptosis rate in HCC cells using flow cytometry; (f). Cell migration ability in HCC cells using Transwell assay. (g) Cell invasion ability in HCC cells using Transwell assay. (h) Stem cell properties of HCC cells using sphere formation assay. (i) The number of colonies formed by HCC cells using colony formation assay. vs. paracancerous tissues; ^# vs. the NC mimic group. All data were expressed as of at least three independent experiments. Data analysis between the two groups was performed by paired -test, and data analysis among multiple groups was performed by two-way ANOVA.

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