FOXA1 Leads to Aberrant Expression of SIX4 Affecting Cervical Cancer Cell Growth and Chemoresistance

Figure 1

FOXA1 is highly expressed in CC and is associated with drug resistance. (a) GEO database analysis of differentially expressed transcription factors in GSE64217 and GSE63514 datasets and Venn map plotting. (b) Expression of FOXA1 in multiple cancers in the GEPIA database. (c) FOXA1 is highly expressed in TCGA-CESC database. (d) High expression of FOXA1 in CESC in TCGA database. (e, f) FOXA1 expression in normal cervical epithelial cells H8 and CC cell lines (HT-3, CaSki, HeLa, and SiHa) at mRNA and protein levels by (e) RT-qPCR and (f) Western blot. (g) The IC50 value of cells to DDP by MTT assay to confirm the successful establishment of drug-resistant cell lines. (h, i) Detection of FOXA1 expression in parental cells and drug-resistant cells by (h) RT-qPCR and (i) Western blot. Data among multiple groups were analyzed by one-way or two-way ANOVA followed by Tukey’s post hoc test (; ). The cell experiment was independently repeated three times.

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