FOXA1 Leads to Aberrant Expression of SIX4 Affecting Cervical Cancer Cell Growth and Chemoresistance

Figure 3

Knockdown of FOXA1 inhibits SIX4 expression in CC cells. (a) SIX4 was predicted to be a downstream gene of FOXA1 using hTFtarget database. (b) High expression of SIX4 in CESC in TCGA database. (c) Conserved binding sites for FOXA1. (d) Potential binding sites of SIX4 promoter to FOXA1. (e) SIX4 expression in TCGA-CESC database. (f) Detection of SIX4 mRNA expression in normal cervical epithelial cells H8 and CC cell lines (HT-3, CaSki, HeLa, and SiHa) by RT-qPCR. (g) Detection of SIX4 protein expression in drug-resistant cells after knockdown of FOXA1 measured using Western blot. (h) The effect of low expression of FOXA1 on the Promoter luciferase activity of SIX4 measured using dual-luciferase assay. (i) The ability of anti-FOXA1 to enrich SIX4 promoter sequence examined using ChIP assay. Data among multiple groups were analyzed by one-way or two-way ANOVA followed by Tukey’s post hoc test (, , ). The cell experiment was independently repeated three times.

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