FOXA1 Leads to Aberrant Expression of SIX4 Affecting Cervical Cancer Cell Growth and Chemoresistance

<div>Overexpression of SIX4 partially reverses the inhibitory effect of sh-FOXA1 on CC cell growth and chemoresistance. (a) Detection of mRNA expression of SIX4 in drug-resistant cells after cotransfection by RT-qPCR. (b) The proliferation of cells measured using colony formation assay. (c) The cell viability measured using EdU assay. (d) The invasive ability of cells determined using Transwell assay. (e) The chemoresistance of cells to DDP measured using MTT assay. (f) The protein expression of p-PI3K, PI3K, p-AKT, and AKT in cells examined using Western blot. 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635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,40.505,0)"><path d="M113 -12C146 -12 170 11 170 46C170 78 146 103 114 103S58 78 58 46C58 11 82 -12 113 -12Z"></path></g><g transform="matrix(.013,0,0,-0.013,43.469,0)"><path d="M241 635C89 635 35 457 35 312C35 153 89 -12 240 -12C390 -12 443 166 443 312C443 466 390 635 241 635ZM238 602C329 602 354 454 354 312C354 172 330 22 240 22C152 22 124 173 124 313S148 602 238 602Z"></path></g><g transform="matrix(.013,0,0,-0.013,49.709,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg>).</span> The cell experiment was independently repeated three times.</div>

Analytical Cellular Pathology

fig5

Figure 5

Figure 5: FOXA1 Leads to Aberrant Expression of SIX4 Affecting Cervical Cancer Cell Growth and Chemoresistance 