Depletion of lncRNA MEG3 Ameliorates Imatinib-Induced Injury of Cardiomyocytes via Regulating miR-129-5p/HMGB1 Axis

Figure 2

Effect of knockdown of MEG3 on the viability and apoptosis of H9c2 cells cultured with imatinib. (a) Dataset GSE161151 was downloaded to analyze the differences in lncRNA expression pattern in normal and ischemic myocardial tissues of mice. (b) H9c2 was transfected with si-NC or si-MEG3#1/2, followed by imatinib pretreatment, then hypoxia induction, and finally qRT-PCR was performed to detect MEG3 expression. (c) After H9c2 treatment, a CCK-8 assay was performed to detect the viability of H9c2 cells in each group. (d) A TUNEL assay was performed to detect apoptosis of H9c2 in each group. (e) Apoptosis of H9c2 cells was detected by flow cytometry for each group of H9c2. , , and .

(a)

(b)

(c)

(d)

(e)