Depletion of lncRNA MEG3 Ameliorates Imatinib-Induced Injury of Cardiomyocytes via Regulating miR-129-5p/HMGB1 Axis

Figure 3

MEG3 directly targets miR-129-5p. (a) The downstream targets of MEG3 were predicted by StarBase and LncBase databases, and a Venn diagrams was plotted. (b) The binding site of MiR-129-5p to MEG3 was shown. (c) MiR-NC or miR-129-5p mimics were transfected with MEG3-WT or MEG3-MUT, respectively, into H9c2 cells, and the luciferase activity of each group was detected using a dual-luciferase reporter gene assay. (d) The enrichment of miR-129-5p with MEG3 in H9c2 cells in lgG group or Ago2 group was analyzed by RIP assay. (e) MEG3 overexpression plasmid and si-MEG3#2 were transfected into H9c2 cells, respectively, and then miR-129-5p expression was detected by qRT-PCR. .

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