Depletion of lncRNA MEG3 Ameliorates Imatinib-Induced Injury of Cardiomyocytes via Regulating miR-129-5p/HMGB1 Axis

Figure 5

MEG3 upregulates HMGB1 expression by targeting miR-129-5p. (a) The downstream target genes of miR-129-5p were predicted by StarBase and TargetScan databases, and a Venn diagram was plotted. (b) The binding site between HMGB1 3^′UTR and miR-129-5p is shown. (c) miR-NC or miR-129-5p mimics were transfected with HMGB1-WT or HMGB1-MUT, respectively, into H9c2 cells, and the luciferase activity of each group was detected using a dual-luciferase reporter gene assay. (d) miR-129-5p mimics or miR-129-5p inhibitors were transfected with H9c2, respectively, and HMGB1 expression was detected by the western blot. (e) si-MEG3#2 + miR-129-5p inhibitors were cotransfected with imatinib-cultured H9c2, and HMGB1 expression was detected by the western blot. .

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