miR-432-5p Inhibits the Ferroptosis in Cardiomyocytes Induced by Hypoxia/Reoxygenation via Activating Nrf2/SLC7A11 Axis by Degrading Keap1

Figure 6

miRNA-432-5p attenuates I/R caused myocardial injury in rats through upregulation of Nrf2 in vivo: (a) the level of exogenous miR-432-5p in the heart tissue; (b) representative image of TTC stained heart in the sham group, I/R rats received miR-432-5p negative control (NC-Lipo), and miR-432-5p mimic (miR-432-5p-Lipo), arrows referred to the damage area; (c) H&E staining was performed in heart sections, miR-432-5p mimic alleviated the injury of infarction in rat heart (magnification was 200x); (d–f) miR-432-5p alleviates the injury of infarction in rat heart through decreasing MDA, increases SOD, and the increases ratio of GSH/GSSG in vivo; (g) miR-432-5p mimic attenuates I/R caused lipid peroxidation in cardiac tissues. Positive immunohistochemistry of 4-HNE in miR-432-5p-Lipo decreases in I/R rats (magnification was 200x); (h) representative western blot image of protein expressions of Nrf2, GPX4, Keap1, and SLC7A11 in rats from the sham group, I/R rats received miR-432-5p negative control and miR-432-5p mimic. miR-432-5p caused decreased expression of Keap1 and increased expression of Nrf2, GPX4, and SLC7A11 in the I/R myocardium. , ; N = 6.

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