miR-432-5p Inhibits the Ferroptosis in Cardiomyocytes Induced by Hypoxia/Reoxygenation via Activating Nrf2/SLC7A11 Axis by Degrading Keap1

Table 1

Sequence information.

Gene	Sequence
miR-432-5p NC	5′-CGAUCG CAU CAG CAU CGAUUG C-3′
miR-432-5p mimic	5′-UCUUGGAGUAGGUCAUUGGGUGG-3′
Keap1-WT	F: 5′-CCCAAGCTT ATGCAGCCAGATCCCAGGCC-3′
	R: 5′-CTAGTCTAGA TCAACAGGTACAGTTCTGCT-3′
Keap1-Mut	F: 5’-CCCAGGAAT ATGGACGGATAAGGCAGGCC-3’
	R: 5′-CTAGGCACCGTGCAGATGAAGAGATGTGCT-3′
nc-Nrf2	F: 5′-UUCUCCGAACGUGUCACGUTT-3′
	R: 5′-ACGUGACACGUUCGGAGAATT-3′
si-Nrf2 1	F: 5′-CCCUGUGUAAAGCUUUCAATT-3′
	R: 5′-UUGAAAGCUUUACACAGGGTT-3’
si-Nrf2 2	F: 5′-GGAGCAAGAUUUAGAUCAUTT-3′
	R: 5′-AUGAUCUAAAUCUUGCUCCTT-3′
si-Nrf2 3	F: 5′-GCUUCAGUGAUUCUGAAAUTT-3′
	R: 5′-AUUUCAGAAUCACUGAAGCTT-3′
miR-432-5p primer	F: 5′-AACGAGACGACGACAGACT-3′
	R: 5′-CTT GGAGTAGGTCAT TGG GT-3′
U6 primer	F: 5′-CTCGCTTCGGCAGCACA-3′
	R: 5′-CGCTTCACGAAT TTGCGTGTCAT-3′
GAPDH	F: 5′-AGAAGGCTGGGGCTCATTTG-3′
	R: 5′-AGGGGCCATCCACAGTCTTC-3′