DNM3OS Enhances the Apoptosis and Senescence of Spermatogonia Associated with Nonobstructive Azoospermia by Providing miR-214-5p and Decreasing E2F2 Expression

Figure 2

Validation of lncRNAs from microarray data. (a) Five upregulated and downregulated lncRNAs selected from the 20 top differentially expressed lncRNAs were analyzed for their expression levels in TCAM-2 cells undergoing treatment with LPS or ADR alone. (b) The levels of DNM3OS, NEAT1, and SNHG1 expression in GC-1 spg cells treated with LPS or ADR alone were determined by quantitative real-time PCR analysis. (c) The expression levels of DNM3OS precursor transcripts, including miR-199a-5p, miR-199a-3p, miR-214-5p, and miR-214-3p, were measured in GC-1 spg cells after treatment with LPS or ADR alone. Data represent the mean value ± SD. ^#, ^##, ^### represents LPS vs. blank; , represents ADR vs. blank.

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