Inhibitory Effect of β-Sitosterol on the Ang II-Induced Proliferation of A7r5 Aortic Smooth Muscle Cells

Figure 3

Effect of β-sitosterol on the apoptosis of Ang II-induced A7r5 cells. (a) A7r5 cells were treated, and apoptosis of A7r5 cells was examined by flow cytometry. (b) Statistical histogram of early and late cell apoptosis rate. (c) Protein expression of pro-caspase 3, cleaved-caspase 3, Bax, and Bcl-2 was examined by western blotting. (d) Pro-caspase 3, cleaved-caspase 3, Bax, and Bcl-2 protein development gray value analysis histogram. Data indicate the mean ± SD (n = 5, n: number of the sample). Significant differences in different groups compared with the control group (without treatment with Ang II or β-sitosterol) are expressed as , , and . Significant differences in different groups compared with the Ang II-alone treatment group are expressed as ^#, ^###, and ^###.

(a)

(b)

(c)

(d)