Suppression of lncRNA OIP5-AS1 Attenuates Apoptosis and Inflammation, and Promotes Proliferation by Mediating miR-25-3p Expression in Lipopolysaccharide-Induced Myocardial Injury

Figure 2

OIP5-AS1 is up-regulated by LPS in myocardial tissues, and knockdown of OIP5-AS1 suppresses LPS-induced inflammation in rats. (a) Relative expression of OIP5-AS1 in myocardial tissues of LPS-induced rats was determined by qRT-PCR. versus sham. (b) The efficiency of OIP5-AS1 knockdown in myocardial tissues was determined by qRT-PCR. **versus control. (c–f) The levels of IL-6, TNF-α, CK-MB, and cTnl in the serum of LPS-induced rats were measured by ELISA. **versus sham. ^##versus LPS + sh-NC. (g) H&E was used to evaluate morphological changes in myocardial tissues of LPS-induced rats. Black, white, and yellow arrows indicated eosinophils, neutrophils, and lymphocytes, respectively. Scale bar = 50 μm. Magnification: 200×. (h) TUNEL staining was performed to determine the myocardial apoptosis in LPS-induced rats. Magnification: 400×.

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