Low-Density Lipoprotein Contributes to Endometrial Carcinoma Cell Proliferation, Migration, and Invasion by Activating the JAK–STAT Signaling Pathway

Figure 2

LDL treatment promoted EC cell proliferation, migration, and invasion in a dose-dependent manner. Ishikawa and RL95-2 cells were stimulated with 0.5, 1.5, and 3.0 μg/mL of LDL for 24 hr. (a) LDL concentrations in the culture media of EC cells were determined by ELISA. (b) The levels of JAK2, p-JAK2, STAT3, and p-STAT3 proteins in EC cells were detected by western blotting. (c) p-JAK2 and p-STAT3 expression were determined by immunofluorescence staining. (d) CCK-8 assays and (e) EdU staining were performed to assess the viability and proliferative ability of Ishikawa and RL95-2 cells, respectively. (f, g) Transwell assays were used to evaluate the migration and invasion capabilities of Ishikawa and RL95-2 cells. Data are expressed as a mean value ± SD; , , and .

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