Low-Density Lipoprotein Contributes to Endometrial Carcinoma Cell Proliferation, Migration, and Invasion by Activating the JAK–STAT Signaling Pathway

Figure 3

LDLs enhanced the proliferation, migration, and invasion of EC cells by regulating the JAK/STAT signaling pathway. Ishikawa and RL95-2 cells were treated with a JAK2 inhibitor (SD-1029), followed by 24 hr of stimulation with 3.0 μg/mL LDL. (a) The concentrations of LDL in the culture media of EC cells were determined by ELISA. (b) The levels of JAK2, p-JAK2, STAT3, and p-STAT3 proteins in EC cells were detected by western blotting. (c) p-JAK2 and p-STAT3 expression were determined by immunofluorescence staining. (d) CCK-8 assays and (e) EdU staining were used to assess the viability and proliferative ability of Ishikawa and RL95-2 cells, respectively. (f, g) Transwell assays were performed to evaluate the migration and invasion abilities of Ishikawa and RL95-2 cells. Data are expressed as a mean value ± SD; and .

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