Mesenchymal Stem Cell-Derived Exosomal miRNA-222-3p Increases Th1/Th2 Ratio and Promotes Apoptosis of Acute Myeloid Leukemia Cells

Figure 1

BM-MSC exosomes increase Th1/Th2 ratio and promote AML cell apoptosis. (a) BM-MSC exosomes were isolated from patients with AML and healthy control individuals, and the phenotype of exosomes was detected by TEM. (b) NTA assay was applied to examine the exosome size (normal: 139.8 nm and AML: 140.5 nm). (c) The protein expression of exosome markers CD6, CD9, and Tsg101 was determined by western blot in the normal group and the AML group. (d) The Th1/Th2 ratio was detected by flow cytometry affected by BM-MSC exosomes from both AML patients and healthy individuals. (e) ELISA was used to detect the contents of IFN-γ and IL-4 affected by BM-MSC exosomes from AML patients and healthy individuals. (f) The apoptosis of AML cell was detected by flow cytometry treatment with BM-MSC exosomes from AML patients and healthy individuals. (g) The caspase 3 protein level changes affected by BM-MSC exosomes from AML patients and healthy individuals. β-actin acts as control. *, ***, ****.

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