Mesenchymal Stem Cell-Derived Exosomal miRNA-222-3p Increases Th1/Th2 Ratio and Promotes Apoptosis of Acute Myeloid Leukemia Cells

Figure 2

IRF2 is targeted by miR-222-3p. (a) StarBase was used for the prediction of miRNAs targeting IRF2 and potential binding sites between miR-222-3p and IRF2 3 UTR. (b) The expression of miR-222-3p in BM-MSC exosomes from patients with AML and the exosomes from healthy control individuals was detected by RT-qPCR. (c) The binding sites between miR-222-3p and IRF2 3 UTR. (d) Dual luciferase reporter assay was applied to examine the relative luciferase activity of IRF2 WT or IRF2 MUT in the mimic NC group and the miR-222-3p mimic group. (e) The protein expression of IRF2 affected by miR-222-3p was determined by western blot in the exosome group, the exosome + mimic NC group, the exosome + miR-222-3p mimic group, the exosome + inhibitor NC group, and the exosome + miR-222-3p inhibitor group. β-actin acts as control. ***, ****.

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