Luteolin Pretreatment Ameliorates Myocardial Ischemia/Reperfusion Injury by lncRNA-JPX/miR-146b Axis

Figure 3

JPX negatively regulates miR-146b expression in HL-1 cells. (a) Plasmid construction map. The predicted binding sequence of miR-146b to JPX was denoted by a short vertical line, and a JPX-WT sequence and a JPX-MUT sequence, respectively, were inserted into the dual luciferase reporter gene plasmids. (b, c) Dual luciferase reporter gene illustrated that JPX could bind to miR-146b. The WT/MUT plasmid containing JPX was cotransfected with miR-NC/miR-146b mimics into HL-1 cardiomyocytes, and 24 and 48 hr after transfection cells were collected for dual luciferase reporter gene detection. Data are presented as mean ± SEM (n = 3). vs. NC + JPX-WT. (d) RT-qPCR verified that JPX regulated miR-146b expression. Data are presented as mean ± SEM (n = 3). , vs. Con, ^@@@ vs. H/R.

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