Luteolin Pretreatment Ameliorates Myocardial Ischemia/Reperfusion Injury by lncRNA-JPX/miR-146b Axis

Figure 4

Expressions of apoptosis proteins and changes in cardiomyocyte apoptosis in HL-1 cells. (a–d) Western blot was used to analyze the expression level of cleaved caspase9, caspase9, cleaved caspase3, caspase3, Bcl-2, Bax, and BAG-1. (e) Annexin V-APC/7-AAD staining followed by flow cytometry was used to detect cardiomyocyte apoptosis in each group. (f) Hoechst 33342 staining was utilized to ascertain cell apoptosis in each group. vs. Con; ^#, ^##, ^### vs. H/R; ^$$, ^$$$ vs. Lut; ^&&, ^&&& vs. Ad-EGFP; ^@@@ vs. Ad-JPX; ^%%% vs. Ad-JPX + Lut; and ^{^^^} vs. Ad-JPX-shRNA.

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