LncRNA AC040162.3 Promotes HCV-Induced T2DM Deterioration through the miRNA-223-3p/NLRP3 Molecular Axis

Figure 2

LncRNA AC040162.3 affects cell proliferation, apoptosis, and pyrolysis in HCV–MIN6. (a) The relative expression of LncRNA AC040162.3 in MIN6 cells transfected with si-AC040162.3-1, si-AC040162.3-2, and si-AC040162.3-3. (b) Insulin concentrations were measured by ELISA. (c) MTT assay was used to determine the cell proliferation rate. (d) The levels of Cleaved caspase-3, Bax, and Bcl₂ were detected by Western blotting. (e) Flow cytometry analysis of cell death. (f) The levels of Caspase-1, IL-18, and IL-1β were detected by Western blotting. (g) TUNEL-determined cell pyrolysis (scale bar = 100 μm). **, ***, vs. si-NC. #, ##, ###, vs. HCV.

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