LINC00659 Inhibits Hepatocellular Carcinoma Malignant Progression by Blocking Aerobic Glycolysis through FUS Recruitment and SLC10A1 Modulation

Figure 4

LINC00659 stabilized SLC10A1 mRNA by recruiting FUS. (a) The Venn diagram of LINC00659 RBP and SLC10A1 RBP. (b) The enrichment of LINC00659 in HepG2 and HuH-7 cells was determined by RIP assay. (c) The enrichment of SLC10A1 3′UTR in HepG2 and HuH-7 cells was determined by RIP assay. (d) The interaction of FUS and LINC00659 in HepG2 and HuH-7 cells was assessed by RNA pull-down assay. (e) The interaction of FUS and SLC10A1 3′UTR in HepG2 and HuH-7 cells was assessed by RNA pull-down assay. (f) The expression of SLC10A1 in HepG2 and HuH-7 cells transfected with sh-FUS was examined by qRT-PCR. (g) The protein level of SLC10A1 in HepG2 and HuH-7 cells transfected with sh-FUS was examined by western blot. (h) The stability of SLC10A1 mRNA was detected by qRT-PCR in HepG2 and HuH-7 cells transfected with sh-FUS after actinomycin D treatment. (i) Relative enrichment of SLC10A1 3′UTR in HepG2 and HuH-7 cells transfected with pcDNA3.1-LINC00659/pcDNA3.1 was assessed by RIP assay. **.

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