Nicotine Suppresses Phagocytic Ability of Macrophages by Regulating the miR-296-3p–SIRPα Axis

Figure 4

Nicotine inhibits the phagocytic ability of macrophages via regulating the miR-296-3p–SIRPα axis. (a) miR-296-3p expression was analyzed by qRT-PCR in U937-derived macrophages treated by DMSO or nicotine for 24 hours. (b and c) 24 hours after U937-derived macrophages were transfected with negative control (NC) or miR-296-3p mimics, nicotine (1 μM) was used to treat the cells for 24 hours. The levels of SIRPα mRNA and protein were analyzed by qRT-PCR and western blotting, respectively. GAPDH serves as an internal control. (d) The phagocytic ability of U937-derived macrophages was analyzed. (e and f) Bars and pictures show SIRPα mRNA and protein levels in U937-derived macrophages transfected with NC or miR-296-3p inhibitor in the presence of DMSO or nicotine. (g) Bars show the phagocytic ability of U937-derived macrophages transfected with NC or miR-296-3p inhibitor in the presence of DMSO or nicotine. * and **.

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