Nicotine Suppresses Phagocytic Ability of Macrophages by Regulating the miR-296-3p–SIRPα Axis

Figure 5

Nicotine decreases miR-296-3p expression by upregulating c-Myc. (a) Bars show c-Myc mRNA expression in U937-derived macrophages transfected with negative control (NC), c-Myc siRNA1, or c-Myc siRNA2, detected by qRT-PCR, and ACTB served as the internal reference. (b) Pictures show c-Myc protein levels in U937-derived macrophages transfected with NC, c-Myc siRNA1, or c-Myc siRNA2, detected by western blot, and GAPDH served as the internal reference. (c) Bars show miR-296-3p expression in U937-derived macrophages transfected with NC, c-Myc siRNA1, or c-Myc siRNA2, detected by qRT-PCR, and ACTB served as the internal reference. (d) Bars show c-Myc mRNA expression in U937-derived macrophages in the presence of DMSO or nicotine, detected by qRT-PCR, and ACTB served as the internal reference. (e) Pictures show c-Myc protein levels in U937-derived macrophages in the presence of DMSO or nicotine, detected by western blot, and GAPDH served as the internal reference. (f) Bars show miR-296-3p expression in U937-derived macrophages transfected with NC or c-Myc siRNA2 in the presence of DMSO or nicotine. (g) Bars show the phagocytosis of U937-derived macrophages transfected with NC or c-Myc isRNA2 in the presence of DMSO or nicotine. *, **, ***, and ****.

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