Selenium Donor Inhibited Hepatitis B Virus Associated Hepatotoxicity via the Apoptosis and Ferroptosis Pathways

Figure 2

Effects of Na₂SeO₃ on HBV replication in HepG2.2.15 cells. (a) HepG2.2.15 cells were transiently transfected with plasmid pcDNA3.1-cIAPs or pcDNA3.1-NC as empty control for 24 hr, and then were treated with Na₂SeO₃ at 5 μM for another 24 hr, and then the cells were harvested and HBV DNA, HBV mRNA HBsAg, and HBeAg quantities were determined. (b) HepG2 cells were transiently transfected with reporter vectors of HBV promoters CP-Luc, XP-Luc, SPI-Luc, and SPII-Luc, along with plasmid pcDNA3.1-cIAPs or pcDNA3.1-NC for 24 hr, and then were treated with Na₂SeO₃ at 5 μM for another 24 hr, and then the luciferase assay was performed. (c) The histogram presents the statistical values of apoptosis rate. (d) HepG2.2.15 cells were treated with Na₂SeO₃ at 5 μM for 24 hr, and then the cells were harvested and the cIAPs levels were determined by western blotting assay. ; ; .

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