Selenium Donor Inhibited Hepatitis B Virus Associated Hepatotoxicity via the Apoptosis and Ferroptosis Pathways

Figure 6

Na₂SeO₃-GPX4 axis inhibits the HBx-related hepatotoxicity through ferroptosis dependent manner. (a and b) HL7702 cells were transduced with leti-HBx or leti-NC as empty control for 24 hr, and then were treated with (a) 50 mM D-GalN or (b) Erastin at 10 μM, along with Na₂SeO₃ at 500 nM for 6 hr, and then the cells were harvested and the GPX4 and ACSL4 levels were determined by western blotting assay. (c and d) HL7702 cells were transduced with leti-HBx or leti-NC as empty control for 24 hr, and then were treated with (c) 50 mM D-GalN or (d) Erastin at 10 μM, along with Na₂SeO₃ at 500 nM, with or without RSL3 at 3 μM for 6 hr, and then the cell viability was monitored by MTT assay. (e) Comparing the serum GPX4 levels among HBV⁺-HCC patients, CHBs patients and the healthy controls. (f) Correlation between the serum selenium levels and serum GPX4 levels in HBV⁺-HCC patients. ; ; .

(a)

(b)

(c)

(d)

(e)

(f)