LINC01614 Promotes Colorectal Cancer Cell Growth and Migration by Regulating miR-217-5p/HMGA1 Axis

Figure 5

LINC01614 promotes CRC progression through the miR-217-5p/HMGA1 axis. (a) qRT-PCR measurements to determine the LINC01614 level in HT29 and SW620 cells transfected with empty vector or OE-LINC01614. (b) mRNA levels of HMGA1 in HT29 and SW620 cells transfected with miR-NC, miR-217-5p mimics, or miR-217-5p mimics+OE-LINC01614. (c and d) CCK8 and colony formation assay evaluating the cell proliferation of HT29 and SW620 cells transfected with miR-NC, miR-217-5p mimics, or miR-217-5p mimics+OE-LINC01614. (e and f) Transwell assay examining the migration and invasion of HT29 and SW620 cells transfected with miR-NC, miR-217-5p mimics, or miR-217-5p mimics + OE-LINC01614. (g) Western blot analysis detecting the effect of miR-NC, miR-217-5p mimics, and miR-217-5p mimics+OE-LINC01614 on the expression of HMGA1, E-cadherin, and N-cadherin. (h) Cell cycle was verified by cell cycle assays in HT29 and SW620 cells transfected with miR-NC, miR-217-5p mimics, or miR-217-5p mimics+OE-LINC01614. Data are presented as the mean ± SD. **.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)