<i>PCSK9-</i>D374Y Suppresses Hepatocyte Migration through Downregulating Free Cholesterol Efflux Rate and Activity of Extracellular Signal-Regulated Kinase

<div>Cholesterol efflux assay in HepG2 cells. (a) Relationship between NBD-cholesterol incubation time and FI. (b) Relationship between HDL treatment time and cholesterol efflux. HDL concentration used in (a) and (b) is 10 <i>μ</i>g/ml. (c) Relationship between HDL concentration and cholesterol efflux. HDL treatment time is 2 h. Wild-type HepG2 cells are used for (a)–(c). (d) Effect of <i>PCSK9</i> overexpression on cholesterol labeling in HepG2 cells. NC: pcDNA3.1; D374Y: pcDNA3.1-<i>PCSK9</i>-D374Y; FI: fluorescence intensity. At least three independent experiments were conducted with triplicate items. 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Analytical Cellular Pathology

fig2

Figure 2

Figure 2: <i>PCSK9-</i>D374Y Suppresses Hepatocyte Migration through Downregulating Free Cholesterol Efflux Rate and Activity of Extracellular Signal-Regulated Kinase 

Figure 2 | PCSK9-D374Y Suppresses Hepatocyte Migration through Downregulating Free Cholesterol Efflux Rate and Activity of Extracellular Signal-Regulated Kinase 